non fat dry milk tbst blotto Search Results


non fat milk tris buffered saline  (Bio-Rad)
98
Bio-Rad non fat milk tris buffered saline
Non Fat Milk Tris Buffered Saline, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non fat milk  (Proteintech)
96
Proteintech non fat milk
Non Fat Milk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tween tbs buffer tbst  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology tween tbs buffer tbst
Tween Tbs Buffer Tbst, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non fat milk tbst  (Thermo Fisher)
99
Thermo Fisher non fat milk tbst
Non Fat Milk Tbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris buffered 148 saline  (Thermo Fisher)
99
Thermo Fisher tris buffered 148 saline
Tris Buffered 148 Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non fat milk in tbst  (Abcam)
99
Abcam non fat milk in tbst
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Non Fat Milk In Tbst, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non fat dry milk in tbst  (Bio-Rad)
99
Bio-Rad non fat dry milk in tbst
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Non Fat Dry Milk In Tbst, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cent non fat dry milk in tbst  (Thermo Fisher)
99
Thermo Fisher cent non fat dry milk in tbst
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Cent Non Fat Dry Milk In Tbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paraformaldehyde  (Rockland Immunochemicals)
93
Rockland Immunochemicals paraformaldehyde
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Paraformaldehyde, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
paraformaldehyde - by Bioz Stars, 2026-08
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blocking solution  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology blocking solution
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Blocking Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-rabbit igg conjugated horseradish peroxidase  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH goat anti-rabbit igg conjugated horseradish peroxidase
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Goat Anti Rabbit Igg Conjugated Horseradish Peroxidase, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rabbit igg hrp conjugated antibody  (Danaher Inc)
99
Danaher Inc rabbit igg hrp conjugated antibody
The MHC region on the short arm of human chromosome 6, contains <t>the</t> <t>HLA-DR</t> (HLA-DRA and HLA-DRB) molecules. The allelic variants of <t>HLA-DRB1</t> ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Rabbit Igg Hrp Conjugated Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The MHC region on the short arm of human chromosome 6, contains the HLA-DR (HLA-DRA and HLA-DRB) molecules. The allelic variants of HLA-DRB1 ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: The MHC region on the short arm of human chromosome 6, contains the HLA-DR (HLA-DRA and HLA-DRB) molecules. The allelic variants of HLA-DRB1 ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques:

A) Heat map of differentially expressed genes (log transformed normalized gene counts) in PBMCs of HLA-DRB1 SE-positive [*04 ( n = 17)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. Genes shown in red have higher gene counts and those shown in blue have lower gene counts. B) HLA-DRB1 gene expression in PBMCs of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. C) HLA-DRB1 gene expression in PBMCs of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 6)] and SE-negative [*03:01/*15:01 ( n = 6)] individuals. FC: fold change, ns: non-significant adjusted P -value.

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: A) Heat map of differentially expressed genes (log transformed normalized gene counts) in PBMCs of HLA-DRB1 SE-positive [*04 ( n = 17)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. Genes shown in red have higher gene counts and those shown in blue have lower gene counts. B) HLA-DRB1 gene expression in PBMCs of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. C) HLA-DRB1 gene expression in PBMCs of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 6)] and SE-negative [*03:01/*15:01 ( n = 6)] individuals. FC: fold change, ns: non-significant adjusted P -value.

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques: Transformation Assay, Expressing

A) Heat map of differentially expressed genes (log transformed normalized gene counts) in CD4+ T cells of HLA-DRB1 SE-positive [*04 ( n = 14)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. B) Heatmap of differentially expressed genes (log transformed normalized gene counts) in CD8+ T cells of HLA-DRB1 SE-positive [*04 ( n = 13)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 8)] individuals. In both heatmaps, genes shown in red have higher gene counts and those shown in blue have lower gene counts. C) HLA-DRB1 gene expression in CD4+ T cells of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 4)] and SE-negative [*03:01/*15:01 ( n = 5)] individuals. D) HLA-DRB1 gene expression in CD8+ T cells of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 3)] and SE-negative [*03:01/*15:01 ( n = 4)] individuals. FC: fold change, ns: non-significant adjusted P -value.

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: A) Heat map of differentially expressed genes (log transformed normalized gene counts) in CD4+ T cells of HLA-DRB1 SE-positive [*04 ( n = 14)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. B) Heatmap of differentially expressed genes (log transformed normalized gene counts) in CD8+ T cells of HLA-DRB1 SE-positive [*04 ( n = 13)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 8)] individuals. In both heatmaps, genes shown in red have higher gene counts and those shown in blue have lower gene counts. C) HLA-DRB1 gene expression in CD4+ T cells of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 4)] and SE-negative [*03:01/*15:01 ( n = 5)] individuals. D) HLA-DRB1 gene expression in CD8+ T cells of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 3)] and SE-negative [*03:01/*15:01 ( n = 4)] individuals. FC: fold change, ns: non-significant adjusted P -value.

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques: Transformation Assay, Expressing

A) Heat map of differentially expressed genes (log transformed normalized gene counts) in CD14+ monocytes of HLA-DRB1 SE-positive [*04 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 4)] individuals. Genes shown in red have higher gene counts and those shown in blue have lower gene counts for the contrast SE+ vs. SE-. B) HLA-DRB1 gene expression in CD14+ monocytes of HLA-DRB1 SE-positive [*04 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 4)] individuals. FC: fold change, ns: non-significant adjusted P -value.

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: A) Heat map of differentially expressed genes (log transformed normalized gene counts) in CD14+ monocytes of HLA-DRB1 SE-positive [*04 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 4)] individuals. Genes shown in red have higher gene counts and those shown in blue have lower gene counts for the contrast SE+ vs. SE-. B) HLA-DRB1 gene expression in CD14+ monocytes of HLA-DRB1 SE-positive [*04 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 4)] individuals. FC: fold change, ns: non-significant adjusted P -value.

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques: Transformation Assay, Expressing

A) HLA-DRB1 gene expression in whole blood samples of HLA-DRB1 SE-positive [*04 ( n = 25)] and SE-negative [*03, *07, and *15 ( n = 44)] individuals. B) HLA-DRB1 gene expression in whole blood samples of individuals carrying different combinations of HLA-DRB1 alleles [*03/*03 ( n = 6), *03/*04 ( n = 5), *03/*07 ( n = 7), *03/*15 ( n = 17), *04/*04 ( n = 7), *04/*07 ( n = 6), *04/*15 ( n = 7), *07/*15 ( n = 13), and *15/*15 ( n = 1)]. *: Adjusted P -value (FDR) < 0.05.

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: A) HLA-DRB1 gene expression in whole blood samples of HLA-DRB1 SE-positive [*04 ( n = 25)] and SE-negative [*03, *07, and *15 ( n = 44)] individuals. B) HLA-DRB1 gene expression in whole blood samples of individuals carrying different combinations of HLA-DRB1 alleles [*03/*03 ( n = 6), *03/*04 ( n = 5), *03/*07 ( n = 7), *03/*15 ( n = 17), *04/*04 ( n = 7), *04/*07 ( n = 6), *04/*15 ( n = 7), *07/*15 ( n = 13), and *15/*15 ( n = 1)]. *: Adjusted P -value (FDR) < 0.05.

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques: Expressing

A) HLA-DRB1 gene expression in whole blood samples of HLA-DRB1 SE-positive [*04 ( n = 44)] and SE-negative [*03, *07, and *15 ( n = 21)] RA patients. B) HLA-DRB1 gene expression in whole blood samples of RA patients carrying different combinations of HLA-DRB1 alleles [*03/*03 ( n = 4), *03/*04 ( n = 7), *03/*07 ( n = 3), *03/*15 ( n = 6), *04/*04 ( n = 12), *04/*07 ( n = 7), *04/*15 ( n = 18), *07/*15 ( n = 2), and *15/*15 ( n = 6)]. *: Adjusted P -value (FDR) < 0.05.

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: A) HLA-DRB1 gene expression in whole blood samples of HLA-DRB1 SE-positive [*04 ( n = 44)] and SE-negative [*03, *07, and *15 ( n = 21)] RA patients. B) HLA-DRB1 gene expression in whole blood samples of RA patients carrying different combinations of HLA-DRB1 alleles [*03/*03 ( n = 4), *03/*04 ( n = 7), *03/*07 ( n = 3), *03/*15 ( n = 6), *04/*04 ( n = 12), *04/*07 ( n = 7), *04/*15 ( n = 18), *07/*15 ( n = 2), and *15/*15 ( n = 6)]. *: Adjusted P -value (FDR) < 0.05.

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques: Expressing

A) Representative western blots showing differential binding of different HLA-DRB antibodies (ab133578, DA2, ab202343, ab98108, and ab168534) to the recombinant proteins HLA-DRB1*03:01, HLA-DRB1*04:01, HLA-DRB1*15:01, and HLA-DRB5*01:01. B) Representative western blot of determining HLA-DRB levels using the antibody DA2 in PBMC samples of individuals carrying HLA-DRB1 *03:01/*04:01 versus HLA-DRB1 *03:01/*15:01 alleles. Vinculin was used as a protein loading control. C) Standard curve of the recombinant protein HLA-DRB1*04:01 detected by the antibody DA2. D) Standard curve of the recombinant protein HLA-DRB1*15:01 detected by the antibody DA2. E) Interpolated HLA-DRB protein levels in individuals carrying HLA-DRB1 *03:01/*04:01 and HLA-DRB1 *03:01/15:01 alleles.

Journal: medRxiv

Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients

doi: 10.1101/2021.01.06.20249032

Figure Lengend Snippet: A) Representative western blots showing differential binding of different HLA-DRB antibodies (ab133578, DA2, ab202343, ab98108, and ab168534) to the recombinant proteins HLA-DRB1*03:01, HLA-DRB1*04:01, HLA-DRB1*15:01, and HLA-DRB5*01:01. B) Representative western blot of determining HLA-DRB levels using the antibody DA2 in PBMC samples of individuals carrying HLA-DRB1 *03:01/*04:01 versus HLA-DRB1 *03:01/*15:01 alleles. Vinculin was used as a protein loading control. C) Standard curve of the recombinant protein HLA-DRB1*04:01 detected by the antibody DA2. D) Standard curve of the recombinant protein HLA-DRB1*15:01 detected by the antibody DA2. E) Interpolated HLA-DRB protein levels in individuals carrying HLA-DRB1 *03:01/*04:01 and HLA-DRB1 *03:01/15:01 alleles.

Article Snippet: Primary antibodies were diluted in 5% non-fat milk in TBST (ab133578 – rabbit monoclonal to HLA Class II DRB1 (1:5000 (Abcam, Amsterdam, the Netherlands)), DA2 – mouse monoclonal to HLA-DRB (1:1000 (Santa Cruz Biotechnology, Heidelberg, Germany)), ab202343 – rabbit polyclonal to HLA Class II DRB1 (1:4000 (Abcam)), ab98108 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), ab168534 – rabbit polyclonal to HLA Class II DRB1 (1:2000 (Abcam)), and E1E9V – rabbit monoclonal to vinculin (1:1000 (Cell Signaling Technology, Solna, Sweden) and incubated overnight at 4°C.

Techniques: Western Blot, Binding Assay, Recombinant